Integrin alpha9 is a 150kD type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes.
It principally associates with the beta1 integrin.
Alpha9beta1 binds to VEGF-C and D, and osteopontin.
The 951 amino acid (aa) extracellular domain of mouse integrin alpha9 contains three beta-propellor repeats and multiple PheGly-GlyAlaPro repeats.
A potential proteolytic cleavage site in human alpha9 ECD is absolutely conserved in mouse alpha9 ECD (Arg 566-Val 567).
The ECD of mouse integrin alpha9 shares 95% and 89% aa sequence identity to rat and human integrin alpha9, respectively.
Applications:Suitable for use in Immunohistochemistry, Western Blot, Flow Cytometry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml.
Tested on recombinant mouse Integrin alpha9
Flow Cytometry: 2.5ug labels 10e6 cells.
Tested on D3 mouse embryonic stem cells.
Immunohistochemistry: 1-15ug/ml in perfusion fixed frozen sections of mouse liver tissueOptimal dilution determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Integrin alpha9. Shows <5% crossreactivity with recombinant human Integrin alpha1, alpha8, alpha9, alphaL, recombinant mouse Integrin 3, 4, 5, E and M.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to Try31-Val979 from mouse Integin alpha9 expressed in NSO cells (NP_598482).
