Integrin alpha8 is a 170-200kD member of the integrin family of adhesion molecules.
It forms an exclusive noncovalent heterodimer with integrin beta-1.
Alpha-8/beta-1 romotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin.
Mouse integrin alpha-8 is a 1025aa type I transmembrane glycoprotein.
It contains a 969aa extracellular region (aa38-1006) and a 29aa cytoplasmic domain.
One isoform may exist that shows a truncation after Ala675 of the precursor.
In the ECD, mouse Integrin alpha8 shares 96% and 90% aa sequence identity with rat and human Integrin alpha-8 protein, respectively.
Applications:Suitable for use in Direct ELISA, Western Blot, Immunocytochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.25ug/ml using mouse lung tissueSimple Western: 10ug/ml using mouse and rat lung tissueImmunocytochemistry: 5-15ug/ml using immersion fixed 4T1 mouse breast cancer cell line Flow Cytometry: 0.25ug/10e6 cells using 4T1 mouse breast cancer cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Integrin alpha8. Species Crossreactivity: rat. In direct ELISAs and Western Blot, ~30% cross-reactivity with recombinant human Integrin alpha 8 is observed, and <1% cross-reactivity with recombinant human Integrin alpha 5, recombinant mouse Integrin alpha 5, recombinant human Integrin alpha V and recombinant mouse Integrin alpha V is observed.
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to Phe38-Phe1007 from mouse Integrin alpha8, expressed in CHO cells.
