IGFBP-rP10, also known as Kazal-type serine protease inhibitor domain-containing protein 1 and bone and odontoblast-expressed protein 1, belongs to the IFGBP superfamily.
It is a 276 amino acid (aa) residue, 36 kD secreted protein that contains an N-terminal IGFBP domain, a Kazal-type serine protease inhibitor region, a C2-type Ig-like domain and a C-terminal poly-glu segment.
An alternate variant with a 16 aa substitution for the C-terminal 33 residues has also been reported.
IGFBP-rP10 is expressed in multiple tissues including osteoblasts/odontoblasts and is up-regulated during osteoblast differentiation and bone regeneration.
Mouse mature IGFBP-rP10 shares 96% and 87% aa sequence identity with rat and human IGFBP-rP10, respectively.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse IGFBP-rP10.
The detection limit for rmIGFBP-rP10 is approximately 1 ng/lane under non-reducing and reducing conditions.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse IGFBP-rP10.
The detection limit for rmIGFBP-rP10 is approximately 0.5 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by mouse IGFBP-rP10 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Selected for its ability to recognize mouse IGFBP-rP10 in direct ELISAs and Western Blots.
