IGFBP-L1 is a secreted 38 kD protein that shares 43% amino acid sequence identity with IGFBP-7 (IGFBP-rP1, Mac25).
Although it was also referred to as IGFBP-rP4 in one publication, it is distinct from Cyr 61, a CCN family protein also named IGFBP-rP4 at one time.
IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal-type serine protease inhibitor region and a C-terminal Ig-like domain.
Human and mouse mature IGFBP-L1 share 76% amino acid sequence homology.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/mL with the appropriate secondary reagents to detect recombinant human IGFBP-L1.
The detection limit for rhIGFBP-L1 is approximately 5 ng/lane under non-reducing and reducing conditions.
In this format, this antibody shows approximately 10% crossreactivity with rmIGFBP-L1 and less than 1% cross-reactivity with rhIGFBP-2, -3, -4, -5, -6, -7, rmIGFBP-1, and rmIGFBP-rP10.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
Specificity：Selected for use as a detection antibody in human IGFBP-L1 Western Blots.
