IGFBP-L1 is a secreted 38 kD protein that shares 43% amino acid sequence identity with IGFBP-7 (IGFBP-rP1, Mac25).
Although it was also referred to as IGFBP-rP4 in one publication, it is distinct from Cyr 61, a CCN family protein also named IGFBP-rP4 at one time.
IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal-type serine protease inhibitor region and a C-terminal Ig-like domain.
Human and mouse mature IGFBP-L1 share 76% amino acid sequence homology.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human IGFBP-L1.
The detection limit for rhIGFBP-L1 is approximately 2 ng/lane under non-reducing and reducing conditions.
In this format, this antibody shows approximately 15% cross-reactivity with rmIGFBP-L1 and less than 1% cross-reactivity with rhIGFBP-1, -2, -3, -4, -5, -6, -7, and rmIGFBP-rP10.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human IGFBP-L1.
The detection limit for rhIGFBP-L1 is approximately 0.5 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human IGFBP-L1 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Selected for its ability to recognize human IGFBP-L1 in direct ELISAs and Western Blots.
