IGSF4C (Immunoglobulin Superfamily member 4C), also known as Nectin-like 4 (Necl-4) and TSLL2, is a 48-55kD type I transmembrane glycoprotein that is a member of the nectin-like family of IGSF molecules.
It is expressed in the brain, kidney, bladder and prostate.
IGSF4C forms cis homophilic dimers on cell surfaces and mediates cell adhesion through homophilic trans-interactions in a Ca 2+/Mg 2+-independent manner.
IGSF4C may also be a tumor-suppressor candidate in human prostate cancer.
The mature 364 amino acid (aa) molecule contains a 299 aa extracellular domain (ECD) (aa 25-323) with three Ig-like motifs and a short cytoplasmic domain.
There is at least one potential splice variant, which shows an insertion of 15 aa after Tyr309.
Applications:Suitable for use in Flow Cytometry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 2.5ug labels 10e6 cells using A172 human cell line Western Blot: 0.1ug/ml using recombinant Human IGSF4C/SynCAM4Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1903-12S, IgG, H&L (PE) Donkey x GoatI1903-30A, IgG, H&L, X-Adsorbed (FITC) Rabbit x Goat
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human lGSF4C. In direct ELISAs and Western Blots, shows ~10% crossreactivity with recombinant human IGSF4B and less than 1% crossreactivity with recombinant human IGSF4D.
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln25-Tyr323 from the human IGSF4C extracellular domain, expressed in NS0 cells. Species Sequence Homology: Canine and mouse (99%)
