Applications:For use as a secondary developing reagent in immunofluorescence assays including immunohistochemistry, immunocytochemistry, flow cytometry, and other fluorescent immunoassays with unlabeled primary antibodies of sheep origin.
Reagent Preparation:Centrifuge the antibody solution in a microfuge before use.
For most applications, a dilution of 1:200 is recommended.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：donkey
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine and rabbit serum to eliminate crossreactivity.
Form：Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
