Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate.
Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome.
Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase.
A splice variant of this gene has been described.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml Human peripheral blood mononuclear cellsImmunoprecipitation: 25ug/ml detects IDS in conditioned cell culture medium spiked with recombinant human IDS
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human IDS. Species Crossreactivity: ~50% with recombinant mouse IDS in ELISA.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser26-Pro550 of human Iduronate 2-Sulfatase (IDS) expressed in NS0 cells
