HES-4 is a 221 aa, 24 kD member of the HES-family of transcriptional repressors.
HES-4 contains a species- specific bHLH domain (aa 33-93), a specific orange DNA-binding domain (aa 109-150), a 65 aa Proline-rich SH3-binding domain, and a WRPW Groucho/TLE interacting C-terminal motif.
HES-4 is a HES-1 paralog likely to maintain stem cell pluripotency.
In the region used as immunogen, the amino acid sequence of human and canine HES-4 are 80% identical.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human HES-4.
The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions.
In this format, this antibody shows less than 1% crossreactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
Specificity：Selected for use as a detection antibody in human HES-4 Western Blots.
