Hepatocyte Cell Adhesion Molecule (HepaCAM) is a 416 amino acid type I transmembrane glycoprotein that belongs to the immunoglobin superfamily.
HepaCAM forms cis-homodimers on the cell surface and may be involved in modulating cell-matrix interactions, likely by promoting cell spreading and motility.
Human HepaCAM runs as a 48-72 kD protein depending on glycosylation.
It contains a 206 amino acid extracellular domain and a 152 amino acid cytoplasmic domain.
Over the extracellular region used as immunogen (aa 34-242), human HepaCAM is 99% and 98% identical to mouse and canine HepaCAM proteins, respectively.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human HepaCAM affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.
Specificity：Recognizes human HepaCAM.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：NS0-derived recombinant human HepaCAM extracellular domain (aa34-242) Accession # NP_689935
