84-1088-98　Heat Shock Protein 105 (HSP105, HSPH1) 100ug　H1833-02

特徴

• The heat shock proteins (HSPs) are a highly conserved family of stress response proteins.
• HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways.
• Heat Shock Proteins are ubiquitously expressed in all organisms.
• They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation.
• Heat shock protein H1 (HSPH1), also known as HSP105 and HSP110, exists as two isoforms, alpha and beta.
• HSPH1 alpha consists of 858 amino acids whereas HSPH1 beta has 814 amino acids.
• Alpha and beta have calculated molecular weights of 96.4 kD and 92 kD, respectively, but an apparent molecular weight of 105-110 kD in SDS-PAGE.
• HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells.
• HSPH1 alpha and beta isoforms share 94% identity.
• Rat HSPH1 has 92% and 97% identity to human and mouse HSPH1, respectively.
• Applications:Western Blot: An antibody concentration of 0.1ug/ml is recommended.
• Optimal dilutions should be determined by the individual laboratory.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.