84-1088-94　HAND2 (dHAND, Thing-2, Hed, HAND Family Transcription Factor) 100ug　H1779-70

特徴

• HAND2 (also known as dHAND, Thing-2 and Hed) is a 24kD, class B member of the basic helix-loop-helix transcription factors superfamily.
• HAND2 is essential for cardiac morphogenesis and required for vascular development and regulation of angiogenesis, possibly through VEGF signaling.
• HAND2 is also implicated in limb development acting as an upstream regulator of sonic hedgehog.
• Alternative initiation and internal deletions result in at least three potential HAND2 isoforms.
• Human and mouse HAND2 proteins are 100% identical.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1.0ug/ml.
• The detection limit for rhHAND2 and rmHAND2 is approximately 1.3ng/well.
• Western Blot: 0.1-0.2ug/ml.
• The detection limit for rhHAND2 and rmHAND2 is approximately 25ng/lane under non-reducing and reducing conditions
• Immunohistochemistry: 10ug/ml to detect HAND2 in mouse embryos.
• Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA.
• After blocking, cells were incubated with diluted primary antibody followed by NorthernLightsTM 557-coupled anti-goat IgG at RT in the dark.
• Between each step, cells were washed with PBS containing BSA.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute by adding sterile PBS.
• Aliquot and store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.