Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase.
Two isoforms (GSK-3α and GSK-3β) share 85% amino acid identity.
GSK-3α is inhibited by phosphorylation at S21 by Akt and other kinases.
Deregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer's disease, bipolar disorder, and cancer.
Applications:Western Blot: An antibody concentration of 1.0ug/ml is recommended.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol, 0.02% sodium azide.
Specificity：Detects endogenous 51kD human, mouse, and rat GSK-3a phosphorylated at S21 using Western Blot. Does not detect phosphorylated GSK-3b.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Phosphopeptide containing S21 site of GSK-3a
