84-1088-84　GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein) 100ug　G2035-50A

特徴

• Gli1 is a member of the Krupple family of zinc finger proteins.
• Gli1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’.
• Gli1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.
• Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-28C: IgG, H&L (HRP) Pab DkxGtI1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab DkxGtI1904-28K: IgG, H&L (HRP) Pab RbxGtI1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGtI1904-28A IgG, H&L (FITC) DkxGtI1904-28E IgG, H&L, X-Adsorbed (FITC) DkxGtI1904-33E IgG, H&L (FITC) DkxGt
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute with sterile PBS.
• Aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.