FRS2 (FGFR substrate 2; also known as SNT and FRS2-Alpha) is a 70-90kD member of the FRS family of lipid-anchored docking proteins.
It is an intermediary between FGF and TRK receptors and their Ras/MAPK signaling cascades.
Human FRS2 is 512 amino acids (aa) in length.
It contains a membrane-anchoring myristoylation signal (aa 1-6), a PTB domain that interacts with FGF and NGF receptors (aa 13-115), and a C-terminal Tyr-rich region that serves as a docking site for Grb2 and Shp2 (aa 196-471).
One splice form shows a deletion of the C-terminal four amino acids.
Over aa 121-449, human FRS2 is 99% and 94% aa identical to canine and mouse FRS2, respectively.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Direct ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1ug/mlImmunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human liver subjected to antigen retrieval
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human FRS2.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asn121-Asn449 from human FRS2 expressed in E. coli.
