FGF-BP, also known as 17kD heparin-binding growth factor binding protein (HBp17) is a secreted, low affinity heparin binding protein.
It binds reversibly to FGF-1, -2, -7, -10, and -22 and functions to release the FGFs from the extracellular matrix for interactions with FGF receptors.
Human FGF-BP shares 66% amino acid sequence homology with the mouse protein.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human FGF-BP.
The detection limit for rhFGF-BP is approximately 2 ng/lane under non-reducing and reducing conditions.
In this format, this antibody shows approximately 5% crossreactivity with rrFGF-BP.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
Specificity：Selected for use as a detection antibody in human FGF-BP Western Blots.
