eIF-4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is an important member of the translation initiation mechanism.
It serves as a scaffold for other initiation factors needed for the recruitment of mRNA to the ribosome, including eIF-4E, eIF-4A, eIF3, and PABP (poly (A)-binding protein).
The eIF-4E/eIF-4G interaction binds to the 5' m7GTP mRNA cap, while the PABP/eIF-4G interaction binds to the poly (A) tail of mRNA.
eIF-4A is an ATP-dependent RNA helicase.
These interactions link the process of poly (A) binding, mRNA cap recognition, and unwinding of secondary structure.
In addition when eIF-4G is cleaved by a viral protease the majority of host cell mediated translation is diverted to an IRES (internal ribosomal entry site) mediated initiation of translation.
Applications:Western Blot: An antibody concentration of 1.0ug/ml is recommended.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 0.02% sodium azide. Reconstitute with 500ul sterile PBS.
Specificity：Detects human eIF-4G1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human eIF-4G1 (rheIF-4G1, aa1400-1599)
