Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml detects ~5ng/lane under reducing and non-reducing conditions
ELISA (Capture): 0.2-0.8ug/ml.
Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, rat EGF antibody and recombinant rat EGF as the standard.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity：Recognizes rat Epidermal Growth Factor.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：E. coli-derived recombinant rat Epidermal Growth Factor
