DNER, also known as brain EGF repeat containing transmembrane protein (BET), is a neuron-specific type I transmembrane notch ligand that is expressed on membranes of dendrites and cell bodies but not axons.
It contains ten extracellular EGF-like domains and one follistatin-like domain (between the 9th and 10th EGF-like domains).
DNER mediates neuron-glia interactions and is required for the functional and morphological maturation of the cerebellum.
The extracellular domains of human and mouse DNER share 89% amino acid sequence homology.
Applications:Suitable for use in ELISA, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 5-15ug/ml (used with immersion fixed A172 human glioblastoma cells)Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a powder lyophilized from a solution containing PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human DNER. In direct ELISAs and Western Blots, 30% crossreactivity with recombinant mouse DNER.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg29-His637 of human DNER expressed in NS0 cell.
