84-1088-66　Developmental Pluripotency-Associated Protein 5 (DPPA5, ECAT2, Esg-1) 100ug　D3221-94T

特徴

• DPPA5, also known as Esg-1 and ECAT2, is a 14kD RNA-binding protein expressed in early embryos, primordial germ cells, and embryonic stem cells and is used as a marker of the pluripotent state.
• Mouse DPPA5 is 118 amino acids in length and shows 63% amino acid identity with human and 81% amino acid identity with rat DPPA5 proteins.
• Applications:Suitable for use in Western Blot, Immunocytochemistry and Direct ELISA.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1ug/ml with the appropriate secondary reagents to detect mouse DPPA5.
• The detection limit for rmDPPA5 is ~5ng/lane under non-reducing and reducing conditions.
• In this format, D3221-94T shows ~25% crossreactivity with rhDPPA5 and ~10% crossreactivity with rmDPPA4.
• D3221-94T has not been optimized for Western Blot detection of DPPA5 in cell lysates
• Immunocytochemistry: 5-15ug/ml detects mouse DPPA5 in mouse embryonic stem cells.
• Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT.
• After blocking, cells were incubated with diluted D3221-94T overnight at 4°C followed by Rhodamine Red™-coupled anti-goat IgG at RT in the dark for 1 hour.
• Between each step, cells were washed with PBS, 0.1% BSA.
• Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse DPPA5.
• The detection limit for rmDPPA5 is ~0.3ng/well.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.