The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively.
PKA R subunits exist as two classes, RI and RII, with each class containing two isoforms, α and β.
Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.
Applications:Western Blot: 1.0ug/ml Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity：Detects endogenous human, mouse, and rat PKA RIb at 45kD using Western Blot. Does not crossreact with recombinant human PKA RIa or PKA RIIb; crossreactivity with PKA RIIa is unknown.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Met1-Val381 of human PKA RI beta, expressed in E. coli.
