XC Chemokine Receptor 1 is a 38kD member of the G-protein coupled receptor 1 family.
It binds XCL1/lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta.
In addition, human herpes virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XC Chemokine Receptor 1.
XC Chemokine Receptor 1 is expressed on neutrophils, CD8+ T cells , NK cells, CD4+ T cells and B cells.
Human XC Chemokine Receptor 1 is a 333aa, 7-transmembrane molecule.
It contains a 32aa N-terminus that lacks glycosylation sites and a 42aa C-terminal cytoplasmic tail.
Over the extracellular regions used for immunization, human XC Chemokine Receptor 1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.
Applications:Suitable for use in Immunocytochemistry, Flow Cytometry, Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry: 15ug/ml Flow Cytometry: 25ug/ml, 10ul labels 1-2.5x10e5 cells in a total reaction volume ≤200ul.
Tested using LPS-treated PBMCs
Western Blot: 0.1-0.2ug/ml.
The detection limit is ~1ng/lane under non-reducing and reducing conditions
Direct ELISA: 0.5-1ug/ml.
The detection limit is ~0.5ng/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human XC Chemokine Receptor 1.
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa1-31, 89-103, 168-190 and 251-267, joined with linker peptides from human XC Chemokine Receptor 1, extracellular N-terminus and loops, expressed in E. coli (UniProt # P46094). Species Sequence Homology: porcine (64%), canine (62%), mouse (54%)
