CRY1 is a critical component of the circadian oscillator that represses CLOCK:BMAL1 mediated transcription when translocated into the nucleus.
It is a member of the DNA photolyase family but has no photolyase activity.
Over the region used as immunogen, human and mouse CRY1 share 94% amino acid sequence homology.
Applications:Suitable for use in Immunocytochemistry, Western Blot, Immunohistochemistry and Direct ELISA.
Other applications have not been tested.
Recommended Dilutions: Western Blot: 0.1ug/ml using Recombinant Human CRY1/Recombinant Mouse CRY1Immunohistochemistry (Frozen): 5-15ug/ml in Perfusion fixed frozen sections of mouse testisImmunocytochemistry: 5-15ug/ml in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C after receipt.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human CRY1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to Lys115-His224 from human CRY1, expressed in E. coli.
