Cyclooxygenase-2 (COX2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70kD microsomal enzyme that belongs to the prostaglandin G/H synthase family.
It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes.
Functionally, COX2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H 2.
Mature human COX2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18-55), a potential membrane interacting region (aa 277-292) and a globular catalytic domain (aa 293-604).
At least one splice form exists that shows an 11 aa substitution for the C-terminal 451 amino acids.
Mature human COX2 shows 87% aa identity to mouse COX2.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml PBS.
Specificity：Recognizes human Cyclooxygenase 2. Species Crossreactivity: Mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：E. coli-derived recombinant human Cyclooxygenase 2 (aa18-604) SwissProt # P35354
