Human CD99 (also known as MIC2 and E2 antigen) is a 32kD Type I, transmembrane glycoprotein expressed in T cells, CD34+ stem cells, monocytes and NK cells.
CD99 contains an extracellular O-glycosylated domain, a transmembrane domain and a short cytoplasmic domain.
Human CD99 has multiple isoforms, one of which involves a deletion of amino acids 34-49 in the extracellular domain.
Over the extracellular region used as immunogen, human CD99 is 42% and 51% identical to the mouse and canine protein sequences, respectively.
Applications:Suitable for use in Immunocytochemistry, Flow Cytometry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 25ug/ml; 10ul will label 1-2.5 x 10e5 cells in a total reaction volume of 200ul.
ELISA (Direct): 0.5-1ug/ml.
The detection limit for rhCD99 is ~0.2ng/well.
Western Blot: 0.1-0.2ug/ml.
The detection limit for rhCD99 is ~0.5ng/lane under non-reducing and reducing conditions
Immunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human CD99. In direct ELISAs and Western Blots, <5% crossreactivity with recombinant mouse CD99.
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa23-122 at the extracellular domain of human CD99, expressed in NS0 cells (P14209).
