CD34 is a 110kD type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucin.
It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells.
Porcine CD34 is 389aa in length and is predicted to have a 272aa extracellular domain (ECD) and a 73aa cytoplasmic tail.
An alternate 307aa splice form with a 4aa substitution for the C-terminal 61aa also exists.
Over aa20-294, porcine CD34 shares 51%, 57% and 62% aa sequence identity with mouse, human, and canine CD34, respectively.
Applications:Suitable for use in Western Blot and Direct ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml Optimal dilutions to be determined by researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes the extracellular domain of porcine CD34. Shows ~20% crossreactivity with rrCD34 and <5% with rmCD34 and rhCD34.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Ala20-Thr294 from the extracellular domain of porcine CD34 expressed in NSO cells with Thr32Ala, Ala52Thr, Pro71Ser, Val126Ala and His278Asn substitutions
