Carboxypeptidase E (CPE) is a peripheral membrane protein that cleaves C-terminal amino acid residues and is involved in the processing of numerous peptide hormones and neurotransmitters.
CPE knockout mice live but become obese due to impaired glucose clearance and insulin resistance.
The CPE precursor includes a signal peptide (aa1-25), a propeptide (aa26-42) and a mature chain (aa43-476).
The amino acid sequence of human CPE is 99.8%, 96.6%, 96.3%, and 90.2% identical to that of chimpanzee, mouse, dog and chicken.
Applications:Suitable for use in Direct ELISA, Western Blot, Immunoprecipitation and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Direct ELISA: 0.5-1.0ug/ml.
The detection limit of recombinant human Carboxypeptidase E is ~1ng/well
Western Blot: 0.1-0.2ug/ml.
The detection for limit recombinant human Carboxypeptidase E is ~5ng/lane under reducing and non-reducing conditions
Immunoprecipitation: Used to immunoprecipitate CPE from conditioned media of transfected NSO cells
Flow Cytometry: 25ug/ml, 10ul of diluted C1209-30 labels 1-5x10e5 cells in a total reaction volume ≤200ul.
Tested on A172 cells.
For intracellular staining cells must first be fixed and permeabilized using 4% paraformaldehyde, 0.1% saponin in PBS.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Carboxypeptidase E. Shows <1% crossreactivity with recombinant human CPA1, CPB1 and recombinant mouse CPA4 in ELISA and Western Blot.
Isotype：IgG
Calc Applications Abbrev：E FC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa42-453 from human Carboxypeptidase E expressed in NSO cells.
