CMG-2, also known as anthrax toxin receptor 2 (ANTXR2) is a 45kD type I membrane protein with a von Willebrand factor A/integrin-like inserted domain in its extracellular region.
CMG-2 binds laminin and collagen type IV.
It also interacts with the protective antigen (PA) subunit of anthrax toxin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml.
The detection limit for rmCMG-2 is approximately 5ng/lane under non-reducing and reducing conditions.
ELISA: 0.5-1ug/ml.
The detection limit for rmCMG-2 is approximately 1.5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse CMG-2. In ELISAs and Western Blots. Shows ~10% crossreactivity with recombinant human CMG-2.Species sequence homology: rat 89% human 84%.
