Cadherins are a multifunctional family of Ca 2+ -dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms.
The cadherin superfamily comprises over forty proteins which are, on average, 50–60% homologous (reviewed in ref 1).
Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion (2) .
Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells.
Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper (3) .
In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins b-catenin and g-catenin which both bind to the actin binding protein a-catenin (4,5) .
The interaction of b-catenin with the cytoplasmic tail of cadherins and other cytoplasmic proteins, including Tcf-family transcription factors and the tumor suppressor protein APC, is thought to be mediated through a region of the b-catenin molecule containing multiple repeats of the 42 amino acid armadillo sequence motif (6) .
In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a significant role in modulating tumor invasion and metastasis (see ref 7 for review).
The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells.
In addition, the tissue specificity of cadherin subtypes are becoming valuable markers for the identification and differential diagnosis of certain cancers (8,9).
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human N-Cadherin.
The detection limit for rhN-Cadherin is approximately 1 ng/lane under non-reducing and reducing conditions.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human N-Cadherin.
The detection limit for rhN-Cadherin is approximately 0.5 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human N-Cadherin affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml is used, the concentration will be 0.5mg/ml.
Specificity：Selected for its ability to recognize human N-Cadherin in direct ELISAs and Western Blots. Shows less than 1% crossreactivity with rhCadherin-8, rhCadherin-11, and rhR-Cadherin.
