The cadherin gene family encodes calcium-dependent adhesion molecules that promote homophilic interactions among cells.
All cadherins are single-pass transmembrane proteins with a variable number of extracellular domains (EC; 110aa long).
Classical cadherins consist of five EC domains and a conserved cytoplasmic domain and are divided in type I and type II subgroups.
Type I comprises cadherin E, N, P and R.
Type II include cadherin-5, -6, -8, -11 and -12.
During embryogenesis differential expression of cadherins can drive morphogenesis by stimulating cell aggregation, defining boundaries between groups of cells and promoting cell migration.
Cadherin-6 seems to be required for the early aggregation of induced mesenchymal cells and their subsequent conversion to epithlium.
Applications:Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml using recombinant human Cadherin-6/KCAD Fc ChimeraImmunocytochemistry: 5-15ug/ml detects Cadherin 6 on MG-63 human osteosarcoma cell lineFlow Cytometry: 0.25ug/1x10e6 cells using MG-63 cell lineOptimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Cadherin 6. In direct ELISAs and Western Blot, shows <5% crossreactivity with recombinant human Cadherin 8, Cadherin 11 and Cadherin 12.
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr22-Ala615 from human Cadherin 6, expressed in NS0 cells. UniProt: P55285.
