Human c-Myc is a 439 amino acid transcription factor with a bHLH/LZ (basic Helix-Loop-Helix, Leucine Zipper) domain.
c-Myc DNA-binding and transcription function is achieved upon heterodimerization with its partner Max.
c-Myc is often over-expressed and mutated in hematopoietic tumors.
Mutations frequently result in truncations that remove the transactivation region or in the bHLH/LZ domain required for association with Max and DNA.
Over the region used as immunogen, human c-Myc is 92% identical to the rat and mouse c-Myc proteins.
Applications:Western Blot: This antibody can be used at 2.0ug/ml with the appropriate secondary reagents to detect human c-Myc.
Immunohistochemistry: This antibody will detect human c-Myc in cells and tissues.
The working dilution is 5-15ug/ml
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human c-Myc.
The detection limit for rhc-Myc is approximately 0.2 ng/well.
ChIP- 5ug/5x10e6 cellsOptimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human c-Myc affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Selected for its ability to recognize human c-Myc in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1% crossreactivity with rhL-Myc and rhN-Myc.
