Brevican (brain-enriched hyaluronan-binding protein) is a 160kD member of the aggrecan/versican proteoglycan family of matrix proteins.
It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins.
Mature human Brevican is 889 amino acids in length.
It contains multiple domains, with an N-terminal V-type Ig-like domain (aa # 52-139) and a C-terminal EGF-like domain (aa # 646-682) and a C-type lectin domain (aa # 695-802).
Multiple isoforms exist where there is an alternate start site at Met228 and a 23 aa substitution for aa # 649-911 that serves as a GPI-anchor motif.
Over aa #23-911, human Brevican shares 82% aa identity with mouse Brevican.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human Brevican affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
