Bone morphogenetic protein 10 (BMP-10) is a TGF-beta superfamily member whose expression is restricted to the developing and postnatal heart.
It is synthesized as a 424 amino acid (aa) pre-proprotein with a putative 21 aa signal peptide, a 295 aa propeptide, and a 108 aa mature protein.
Whereas mature BMP-10 is secreted as a 25kD disulfide-linked homodimer, recombinant BMP-10 propeptide is secreted as a 48kD monomer.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:Recognizes human BMP10. Show <1% crossreactivity with mature recombinant human BMP10 in direct ELISA.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to aa20-313 from human Bone Morphogenetic Protein 10 expressed in NSO cells (O95393). Species Sequence Homology: mouse, 82%.