Bcl-2/adenovirus E1B 19kD protein-interacting protein 3-like (BNIP3L) also known as BNIP3 alpha and NIP3-like protein X (Nix), is a proapoptotic member of BNIP3 protein family.
BNIP3L is a functional homolog of BNIP3 and both proteins contain a single Bcl-2 homology 3 (BH3) domain.
BNIP3L is a 219 amino acid (aa), 24kD (predicted) protein that contains a C-terminal transmembrane domain required for mitochondrial localization, homodimerisation, and regulation of its proapoptotic function.
BNIP3L may antagonize the activity of BCL2 family antiapoptic proteins by directly interactacting with proteins such as viral E1B-19K and cellular Bcl-2 and Bcl-xL.
BNIP3L shares 56% amino acid sequence identity with BNIP3 and 98% with mouse and rat BNIP3L.
Applications:Western Blot: An antibody concentration of 1.0ug/ml is recommended.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with 100ul sterile PBS.
Specificity：Detects endogenous human, mouse, and rat BNIP3L on Western Blots. BNIP3L is detected at an apparent molecular weight of 38kD in Western Blots. Has less than 10% crossreactivity to recombinant human BNIP3L in Western Blots.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：E. coli derived recombinant human BNIP3L (Ser2-Glu184)
