84-1088-36　BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3) 100ug　B2199-95

特徴

• BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3), also known as 19kD interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family.
• BNIP3 is a 194 amino acid, 21.5kD (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function.
• BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19kD protein.
• Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions.
• The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation.
• Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3.
• Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.