Aurora B, a serine/threonine protein kinase, is a key regulator of the onset of cytokinesis and chromosome segregation.
Phosphorylation of Histone H3 at Ser 10 and Ser 28 by Aurora B contributes to chromosome number instability and mitotic chromosome condensation.
In addition, Aurora B phosphorylates MCAK (mitotic centromere-associated kinesin) on Ser 92 and this inhibits MCAK’s microtubule depolymerizing activity.
Aurora B localizes to kinetochore fibers during chromosome movement and segregation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: An antibody concentration of 1.0ug/ml is recommended.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 0.02% sodium azide. Reconstitute with sterile PBS, 40-50% glycerol.
Specificity：Detects human and mouse Aurora B.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：E. coli-derived recombinant human Aurora B (rhAurora B, aa1-150)
