ATG4A (Autophagy related protein 4 homolog A; also Autophagin 2) is a 50kD cytosolic cysteine protease mostly found in skeletal muscle and brain.
It belongs to the Autophagin protein family which is involved in autophagy, a biological process in which endogenous protein and damaged organelles are destroyed.
There is a potential for multiple splice forms, namely isoform 1, isoform 2, and isoform 3.
Human ATG4A shares 92% and 94% aa sequence identity with mouse and canine ATG4A, respectively.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity：Recognizes human ATG4A.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Sf21-derived, recombinant human Autophagy related protein 4 homolog A isoform 1 (aa2-398) UniProt # Q8WYN0
