APC is a ubiquitous, tumor-suppressor protein that is mutated in most colon cancers, generally resulting in a gene product that is truncated in the carboxy terminal region.
APC contains several binding domains.
The region used as an immunogen overlaps the EB1, DLG (PDZ sequence) and PSD-93 domains.
These domains bind microtubules, mitotic spindles and F-actin and are involved in cell cycle, division and migration.
The immunogen does not include the beta-catenin-binding region, which antagonizes Wnt signaling.
The human APC amino acid sequence used as immunogen is 83% and 86% identical to mouse and rat APC, respectively.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Optimal dilutions to be determined by the researcher.
Recommended Dilutions:Western Blot: 0.1-0.2ug/ml, the detection limit for recombinant human APC C-terminus is~1ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 5-15ug/mlELISA: 0.5-1ug/ml, the detection limit for recombinant human APC C-terminus is ~0.2ng/well
Optimal dilutions to be determined by researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognize human APC C-Terminus.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser2537-Val2843 from the C-terminus of human APC, expressed in E. coli
