Annexin A13 (ANXA13), also known as Annexin XIII and Intestine-Specific Annexin, is a member of the Annexin protein family.
Two isoforms have been identified resulting from alternate splicing.
Isoform A consists of 316 amino acids and has a predicted molecular weight of 36kD whereas isoform B is composed of 375 amino acids and has a predicted molecular weight of 40kD.
Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane.
The Annexin’s have a molecular weight of approximately 35 to 40kD and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites.
The C terminal domain is comprised of four 60-70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats).
The four annexin repeats form a highly α-helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner.
Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways.
The specific function of Annexin A13 has not been determined but it is associated with the plasma membrane of undifferentiated, proliferating endothelial cells and differentiated villus enterocytes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes endogenous human and mouse ANXA13 in Western Blots. Does not crossreact with recombinant human Annexin A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, or A11 in Western Blots.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to Met1-His316 from human ANXA13, expressed in E. coli.
