Annexin A7 (ANXA7), also known as Annexin VII and Synexin (SNX), is a 50.3kD member of the Annexin protein family.
The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane.
The Annexin's have a molecular weight of approximately 35-40kD and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites.
The C terminal domain is comprised of four 60-70 amino acid (aa) repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats).
The four annexin repeats form a highly α-helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner.
Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways.
Annexin A7 was identified as a calcium- dependent membrane-binding protein that not only fuses membranes but also acts as a voltage-dependent calcium channel.
Identification of alternate mRNA splicing indicates two Annexin A7 isoforms differing in the N-terminal domain.
Human Annexin A7 shares 92% identity with mouse and rat Annexin A7.
Applications:Western Blot: An antibody concentration of 1.0ug/ml is recommended.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.1ml is used, the concentration will be 1mg/ml.
Specificity：Detects endogenous human, mouse, and rat Annexin A7 on Western Blots. Does not crossreact with recombinant human Annexin A1, A2, A3, A4, A5, A6, A8, A9, A10, A11, or A13 in Western Blots.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：E. coli derived recombinant human Annexin A7 (aa1-466)
