84-1088-25　Annexin A4 (Annexin IV, ANX4, ANXA4, Lipocortin IV, Placental Anticoagulant Protein II, Zymogen Granule Membrane-Associated Protein 36kD, ZAP36) 100ug　A2295-15D

特徴

• Annexin A4 is a 321aa, 36kD member of the Annexin protein family.
• Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane.
• The Annexin's have a molecular weight of ~35-40kD and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites.
• The C terminal domain is comprised of four 60-70aa repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats).
• The four annexin repeats form a highly alpha-helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner.
• Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth and intracellular signal transduction pathways.
• Human Annexin A4 shares 92% identity with mouse and rat Annexin A4.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-28K: IgG, H&L (HRP) Pab RbxGtI1904-53L6: IgG, H&L (HRP) Pab RbxGtI1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGtI1903-88A6: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS, 0.02% sodium azide.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.