84-1088-23　Angiopoietin-like 3 (ANGPTL3, ANGPT5) (Biotin) 50ug　A2293-48D

特徴

• Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins.
• Human ANGPTL3 contains an N-terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C-terminal fibrinogen-like domain that binds integrin alphavbeta3.
• In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N-terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C-terminal fragment that can induce angiogenesis.
• Full length and cleaved ANGPTL3 circulate in plasma.
• Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.2ug/ml Detection limit for rhANGPLT3 is ~1ng/lane under non-reducing and reducing conditions.
• In this format, A2293-48D shows ~20% cross-reactivity with rmANGPTL3
• Optimal dilutions should be determined by each laboratory for each application.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile TBS, 0.1% BSA.
• Aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.