Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins.
Human ANGPTL3 contains an N-terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C-terminal fibrinogen-like domain that binds integrin αvβ3.
In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N-terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C-terminal fragment that can induce angiogenesis.
Full length and cleaved ANGPTL3 circulate in plasma.
Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human ANGPLT3.
The detection limit for rhANGPTL3 is approximately 1ng/lane under non-reducing and reducing conditions.
In this format, this antibody shows approximately 20% crossreactivity with rmANGPTL3.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human ANGPLT3.
The detection limit for rhANGPTL3 is approximately 2.5ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human ANGPLT3 in direct ELISAs and Western Blots.
