The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species.
Levels of the Actin protein may vary between different cell types, but are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading.
Applications:Suitable for use in Immunocytochemistry and Western Blot.
Stains immersion fixed HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line.
Western Blot: 1ug/ml.
HeLa human cervical epithelial carcinoma cell line, MCF7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC12 rat adrenal pheochromocytoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Actin. Species Crossreactivity: mouse and rat
