Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA).
PKR inhibits translation initiation through phosphorylation of the alpha subunit of the initiation factor eIF2 (eIF2alpha) and also controls the activation of several transcription factors such as NF-kappaB, p53 and Stats.
In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.
Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation at Thr446 and Thr451 in the activation loop.
Substitution of Thr451 to alanine completely inactivates PKR, while a mutant with a Thr446 to alanine substitution was partially active.
PKR phosphorylates the a subunit of eukaryotic initiation factor-2 and inhibits protein synthesis.
It is an important component of the interferon response to virus infection.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000 Detects phospho-PKR (Thr451) in RIPA lysate of IFNgamma and calyculin treated HeLa cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS, pH 7.3, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol
Specificity：Recognizes human phosphorylated PKR (Thr451), Mr 68kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to amino acid region encompassing the human phospho-PKR (Thr451).
