Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c-Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking.
c-Met contains three tyrosines (Tyr-xx-x-Tyr-Tyr motif) within the activation loop of the catalytic domain.
This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation.
With c-Met ( and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation.
Activation of the c-Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin and GAB1) and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-gamma and STAT3).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000 detects phospho-Met (Tyr1230/Tyr1234/Tyr1235) in A431 cells treated with EGF
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes Met phosphorylated on both Tyr1234/Tyr1235 and Tyr1230/Tyr1234/Tyr1235. Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-c-Met (Tyr1230/Tyr1234/Tyr1235). Species sequence homology: rat-100%
