Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quality Control TestingWestern Blot: 0.5ug/mL dilution detected phospho-IRS1 (Ser616) in RIPA lysates from TPA treated CHO cells transfected with human IRS1.
TPA treated lysates from CHO cells transfected with human IRS11 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-IRS1 (Ser616) for 2 hours at room temperature.
Proteins were visualized via HRP and chemiluminescent detection.
Arrow indicates phospho-IRS1.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.2, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide.
Specificity：Recognizes phosphorylated IRS-1(Ser616), Mr 165kD Species Cross-reactivity: Human tested. Predicted to cross-react with Mouse and Rat (100% homologous) based on sequence homology.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-IRS-1 (Ser616),
