84-1087-99　Insulin-Like Growth Factor I Receptor/Insulin Receptor, phosphorylated (Tyr1158, Tyr1162, Tyr1163) (IGF1R, IR) 100ul　I7661-18N

特徴

• Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane tyrosine kinase.
• It is widely expressed among many cell types in fetal and postnatal tissues and many cell lines.
• Upon binding of its ligands, IGF-I and IGF-II, autophosphorylation of receptors occurs.
• The triple tyrosine cluster (Tyr1131, Tyr1135 and Tyr1136) within the kinase domain is the earliest major site of autophosphorylation.
• Phosphorylation of these three tyrosine residues is necessary for activation of kinases.
• Insulin receptors (IRs) share significant similarity with IGF-I receptors in structure and function.
• There is also an equivalent triple tyrosine cluster (Tyr1146, Tyr1150 and Tyr1151) in its activation loop of the kinase domain.
• Tyrosine autophosphorylation of the receptors is one of the earliest cellular responses to insulin stimulation.
• The autophosphorylation begins by phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151.
• The full activation of receptor kinase activity depends on the triple tyrosine phosphorylation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 detected phospho-IR (Tyr1158/Tyr1162/Tyr1163) in RIPA lysate of untreated and insulin treated CHO cells transfected with IR
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.