Lysate from SKBR3 was resolved by electrophoresis, transferred to PVDF, and probed with B2765-01 for 2 hours at RT.
Proteins were visualized via HRP and chemiluminescent detection.
Band observed at ~50kD indicates Brk.
An upper band is thought to be the 73kD ELF-related protein that shares 70% homology with the immunizing peptide
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide.
Specificity：Recognizes human Brk (breast tumor kinase, PTK6) at ~52kD. It can also recognize the 73kD ELF-related protein that shares 70% homology with the immunizing peptide.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the N-terminal region of human Brk, conjugated to KLH.
