84-1087-90　Glucagon (BSA, Azide, Glycerol Free) 50ug　G2040-01K

特徴

• Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide.
• The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2.
• The GLP 1 & 2 stimulates intestinal growth and up regulates villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis.
• The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration
• Glucagon (GLGN) is a 29aa pancreatic hormone (chr 2q36), which together with insulin, is an important regulator of glucose metabolism.
• When in need of glucose, glucagon is secreted by the A cells of the pancreas and binds to target cell-surface receptors in the liver.
• This triggers a series of events that ultimately results in glycogenolysis and gluconeogenesis and a consequent rise in glucose levels in the blood.
• The glucagons in Human, rabbit, rat, porcine and bovine are identical.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1-10ug/ml (ECL)ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well (See G2040-01J)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.